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标题：Analysis of cytoskeletal proteins and Ca2+-dependent regulation of structure in intestinal brush borders from rachitic chicks
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作者：Christine L. Howe ; Thomas C. S. Keller ; Mark S. Mooseker 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1982
卷号：79
期号：4
页码：1134-1138
DOI：10.1073/pnas.79.4.1134
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We have investigated several structural aspects of the intestinal epithelial brush border from rachitic chicks. At both the light and electron microscope levels, rachitic brush borders are indistinguishable from controls. Although several of the prominent periodic acid-Schiff-positive proteins of the brush border membrane have slightly slower mobilities on sodium dodecyl sulfate/polyacrylamide gels than do corresponding proteins from control brush borders, the major components of the microvillus core, including subunits of 105, 95, and 68 kilodaltons, actin, and calmodulin, are not detectably different. As assayed by a 125I-labeled calmodulin gel overlay technique, the same calmodulin-binding proteins are present in rachitic and control brush borders. Two proteins, the 105-kilodalton subunit of the microvillus core and an approximately 30-kilodalton membrane protein, bind calmodulin in a calcium-independent manner. Four cytoskeletal proteins (250, 190, 180, and 150 kilodaltons) and one membrane protein (35 kilodaltons) bind calmodulin only in the presence of calcium. Calcium-dependent solation of microvillus core proteins and calcium-dependent phosphorylation of the 20-kilodalton light chain of brush border myosin both occur as in controls. Our results show that rachintic chicks have brush borders that are quite similar to controls with respect to their ultrastructural organization, constituent contractile proteins, and calcium-dependent regulation of contractility and microvillus core structure. Therefore, the decreased absorption of calcium by intestinal epithelial cells in rachitic chicks is probably not due to gross structural or chemical differences in the brush border cytoskeleton.
关键词：calcium ; cytoskeleton ; intestine ; microvillus ; vitamin D