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标题：Prostaglandin-stimulated GTP hydrolysis associated with activation of adenylate cyclase in human platelet membranes
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作者：H A Lester ; M L Steer ; A Levitzki 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1982
卷号：79
期号：3
页码：719-723
DOI：10.1073/pnas.79.3.719
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：In membranes purified from human blood platelets, basal guanosine triphosphate (GTP) hydrolysis is reduced by a factor of approximately 6 by exposure to N-ethylmaleimide (10 mM). This decreased background enables the detection of an additional GTP hydrolysis in the presence of prostaglandin E1 (PGE1). The PGE1-stimulated GTPase has several properties correlated with PGE1-stimulated adenylate cyclase in this preparation. The two enzymes have similar dose-response relationships (half-maximal stimulation at 0.1 microM PGE1). Exposure to cholera toxin blocks the PGE1-stimulated GTPase and activates adenylate cyclase. Both enzymes are activated by submicromolar concentrations of GTP, although the Km for the GTPase is about 10 times greater than that for the adenylate cyclase. The data are discussed in relation to the hypothesis that hormone-stimulated adenylate cyclase (i) is activated as a regulatory component binds a molecular of GTP and (ii) is deactivated as this molecule is hydrolyzed.