文章基本信息

标题：Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts
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作者：J M Rossi ; D T Burke ; J C Leung 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1992
卷号：89
期号：6
页码：2456-2460
DOI：10.1073/pnas.89.6.2456
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.