文章基本信息

标题：Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae
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作者：R H Schiestl ; T D Petes
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1991
卷号：88
期号：17
页码：7585-7589
DOI：10.1073/pnas.88.17.7585
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：DNA fragments (generated by BamHI treatment) with no homology to the yeast genome were transformed into Saccharomyces cerevisiae. When the fragments were transformed in the presence of the BamHI enzyme, they integrated into genomic BamHI sites. When the fragments were transformed in the absence of the enzyme, they integrated into genomic G-A-T-C sites. Since the G-A-T-C sequence is present at the ends of BamHI fragments, this results indicates that four base pairs of homology are sufficient for some types of mitotic recombination.