文章基本信息

标题：Specific initiation by RNA polymerase I in a whole-cell extract from yeast
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作者：M C Schultz ; S Y Choe ; R H Reeder 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1991
卷号：88
期号：3
页码：1004-1008
DOI：10.1073/pnas.88.3.1004
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A protocol is described for making a soluble whole-cell extract from yeast (Saccharomyces cerevisiae) that supports active and specific transcription initiation by RNA polymerases I, II, and III. Specific initiation by polymerase I decreases in high-density cultures, paralleling the decrease in abundance of the endogenous 35S rRNA precursor. This extract should be useful for studying the molecular mechanisms that regulate rRNA transcription in yeast.