文章基本信息

标题：Thickness determination of biological samples with a zeta-calibrated scanning tunneling microscope.
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作者：Z H Wang ; T Hartmann ; W Baumeister 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1990
卷号：87
期号：23
页码：9343-9347
DOI：10.1073/pnas.87.23.9343
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A single-tube scanning tunneling microscope has been zeta-calibrated by using atomic steps of crystalline gold and was used for measuring the thickness of two biological samples, metal-coated as well as uncoated. The hexagonal surface layer of the bacterium Deinococcus radiodurans with an open network-type structure shows thickness values that are strongly influenced by the substrate and the preparation method. In contrast, the thickness of the purple membrane of Halobacterium halobium with its densely packed less-corrugated structure exhibits very little variation in thickness in coated preparations and the values obtained are in good agreement with x-ray data.