文章基本信息

标题：Purification of two transcription factors required for initiation by mammalian RNA polymerase II
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作者：T Kawaguchi ; S Kitajima ; Y Niho 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1990
卷号：87
期号：17
页码：6619-6623
DOI：10.1073/pnas.87.17.6619
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We have purified two general transcription factors (FA and FE) necessary for specific transcription by mammalian RNA polymerase II to near homogeneity. Both activities are associated with peptides of approximately 33 kDa. FA and FE do not replace one another and show different kinetics of action in a sarkosyl block assay. In particular, FE participated in a rapid reaction after the formation of an initial complex with the other transcription factors. Furthermore, FE can associate with purified calf thymus RNA polymerase II.