文章基本信息

标题：Detection of individual fluorescently labeled reovirions in living cells
本地全文：下载
作者：A Georgi ; C Mottola-Hartshorn ; A Warner 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1990
卷号：87
期号：17
页码：6579-6583
DOI：10.1073/pnas.87.17.6579
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Reovirus serotype 1 (Lang) can be conjugated with rhodamine B or fluorescein isothiocyanate in a way that preserves viral infectivity. We have used epifluorescence microscopy to detect individual virions bound to the surface of cells and to follow in real time the early stages of reovirus infection in living cells. Following uptake of the virus into endocytic vesicles, the movement of these vesicles can be observed readily. The vesicle movement is inhibited by nocodazole or colchicine, consistent with previous findings that the movement of intracellular vesicles is often microtubule-based.