文章基本信息

标题：Method to identify genomic targets of DNA binding proteins
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作者：L Sompayrac ; K J Danna
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1990
卷号：87
期号：9
页码：3274-3278
DOI：10.1073/pnas.87.9.3274
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We have devised a cyclical immunoprecipitation protocol that can be used to identify and clone a specific DNA sequence that is recognized by a DNA binding protein, even if that sequence is present in only one copy in the genome of a mammal. As an example, we have used this procedure to purify mouse genomic sequences to which the simian virus 40 tumor (T) antigen binds.