文章基本信息

标题：Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts
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作者：K R Harkins ; R A Jefferson ; T A Kavanagh 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1990
卷号：87
期号：2
页码：816-820
DOI：10.1073/pnas.87.2.816
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by promoters from photosynthesis-associated genes showed a striking dependence on cell type. These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts.