文章基本信息

标题：Purification of DNA polymerase II stimulatory factor I, a yeast single-stranded DNA-binding protein
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作者：W C Brown ; J K Smiley ; J L Campbell 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1990
卷号：87
期号：2
页码：677-681
DOI：10.1073/pnas.87.2.677
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Incidental to the purification of yeast DNA polymerase II was the observation that various chromatographic fractions contained activities that stimulated synthesis by this polymerase. In this paper we report the purification and initial characterization of one such factor, stimulatory factor I (SFI). SFI, which is associated with an apparent complex of three polypeptides of 66, 37, and 13.5 kDa, binds preferentially to single-stranded DNA, possibly explaining its ability to stimulate DNA polymerase II. Single-stranded DNA-binding activity is associated with the 66-kDa polypeptide.