文章基本信息

标题：A Trim5-cyclophilin A fusion protein found in owl monkey kidney cells can restrict HIV-1
本地全文：下载
作者：Sébastien Nisole ; Clare Lynch ; Jonathan P. Stoye 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2004
卷号：101
期号：36
页码：13324-13328
DOI：10.1073/pnas.0404640101
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Lv1 restriction of HIV-1 in the cells of Old World monkeys is associated with the expression of the Trim5 gene. Uniquely, in owl monkey kidney cells, HIV-1 restriction is dependent on the ability of incoming viral capsid protein to bind cyclophilin A (CypA). Cloning of the owl monkey Trim5 gene now reveals the presence of an inserted CypA pseudogene within intron 7 of the Trim5 gene. This insertion results in the formation of a chimeric Trim5-CypA transcript. Transfer of a cDNA corresponding to this transcript into human cells confers cyclosporin A-sensitive resistance to HIV-1 infection. The restriction factor appears to be a chimeric protein created by retrotransposon-mediated exon shuffling.