文章基本信息

标题：A feedback mechanism regulates monoallelic odorant receptor expression
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作者：Joseph W. Lewcock ; Randall R. Reed
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2004
卷号：101
期号：4
页码：1069-1074
DOI：10.1073/pnas.0307986100
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：In olfactory neurons, expression of a single odorant receptor (OR) from a repertoire of >1,000 genes is required for odor coding and axonal targeting. Here, we demonstrate a role for OR protein as an essential regulator in the establishment of monoallelic OR expression. An OR-promoter-driven reporter expresses in a receptor-like pattern but, unlike a native OR, is coexpressed with an additional OR allele. Expression of a functional OR from the identical promoter eliminates expression of other OR alleles. The presence of an untranslatable OR coding sequence in the mRNA is insufficient to exclude expression of a second OR. Together, these data identify the OR protein as a critical element in a feedback pathway that regulates OR selection.