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标题：CO2, not , facilitates oxidations by Cu,Zn superoxide dismutase plus H2O2
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作者：Stefan I. Liochev ; Irwin Fridovich
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2004
卷号：101
期号：3
页码：743-744
DOI：10.1073/pnas.0307635100
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The Cu,Zn superoxide dismutase catalyzes [IMG]f1.gif" BORDER="0"> -dependent oxidations by H2O2. This activity has been shown to depend on the creation of a bound oxidant at the Cu(II) by interactions with H2O2. The bound oxidant was then thought to oxidize [IMG]f1.gif" BORDER="0"> to [IMG]f2.gif" BORDER="0">, which diffuses into the bulk solution and there oxidizes diverse substrates. We now find that CO2 rather than [IMG]f1.gif" BORDER="0"> facilitates the peroxidations catalyzed by Cu,Zn superoxide dismutase. This fact was shown by a lag in the rate of peroxidation of NADPH when [IMG]f3.gif" BORDER="0"> was added last and by a burst in the rate when aqueous CO2 was added last. Both the lag and the burst were eliminated by carbonic anhydrase.
关键词：carbon dioxide ; bicarbonate ; carbonic anhydrase ; Cu,Zn SOD ; hydrogen peroxide