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  • 标题:Transcriptome-based determination of multiple transcription regulator activities in Escherichia coli by using network component analysis
  • 本地全文:下载
  • 作者:Katy C. Kao ; Young-Lyeol Yang ; Riccardo Boscolo
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:2004
  • 卷号:101
  • 期号:2
  • 页码:641-646
  • DOI:10.1073/pnas.0305287101
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Cells adjust gene expression profiles in response to environmental and physiological changes through a series of signal transduction pathways. Upon activation or deactivation, the terminal regulators bind to or dissociate from DNA, respectively, and modulate transcriptional activities on particular promoters. Traditionally, individual reporter genes have been used to detect the activity of the transcription factors. This approach works well for simple, non-overlapping transcription pathways. For complex transcriptional networks, more sophisticated tools are required to deconvolute the contribution of each regulator. Here, we demonstrate the utility of network component analysis in determining multiple transcription factor activities based on transcriptome profiles and available connectivity information regarding network connectivity. We used Escherichia coli carbon source transition from glucose to acetate as a model system. Key results from this analysis were either consistent with physiology or verified by using independent measurements.
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