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  • 标题:Antibiotic resistance of Escherichia coli isolated from a poultry slaughterhouse
  • 本地全文:下载
  • 作者:Gabriela Gregova ; Marta Kmetova ; Vladimfr Kmet
  • 期刊名称:Annals of Agricultural and Environmental Medicine
  • 印刷版ISSN:1232-1966
  • 电子版ISSN:1898-2263
  • 出版年度:2012
  • 卷号:19
  • 期号:01
  • 页码:75-77
  • 出版社:Institute of Agricultural Medicine in Lublin
  • 摘要:The aim of the study was to investigate the antibiotic resistant E. coli strains isolated from bioaerosols and surface swabs in a slaughterhouse as a possible source of poultry meat contamination. The highest air coliforms contamination was during shackling, killing and evisceration of poultry. The strains showed resistance to ampicillin (89%), ceftiofur (62%) and cefquinome (22%), while resistance to ampicillin with sulbactam was only 6%. Resistance to streptomycin and gentamicin was detected in 43% vs. 14% isolates; to tetracycline 33%; to chloramphenicol and florfenicol in 10% vs. 18% isolates; to cotrimoxazol in 35% isolates; to enrofloxacin in 43 % isolates. The higher MIC of ceftazidime (3.6 mg.l-1) and ceftriaxon (5.2 mg.l-1) revealed the presence of ESBLs in 43% of isolates. From 19 selected phenotypically ESBL positive strains, 16 consisted of CMY-2 genes, while CTX-M genes were not detected by PCR. Maldi tof analysis of selected E. coli showed a clear clonal relatedness of environmental strains from various withdrawals.
  • 关键词:Escherichia coli; antibiotic resistance; ESBL; MALDI ToF; poultry slaughterhouse;
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