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标题：OPTIMIZATION OF INULINASE PRODUCTION USING COPRA WASTE BY Kluyveromyces marxianus var. marxianus
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作者：M. DILIPKUMAR ; M. RAJASIMMAN ; N. RAJAMOHAN 等
期刊名称：Chemical Industry and Chemical Engineering Quarterly
印刷版ISSN：1451-9372
出版年度：2010
卷号：16
期号：04
页码：319-327
出版社：Association of the Chemical Engineers
摘要：Kluyveromyces marxianus var. marxianus was found to secrete a large amount of extracellular inulinase in to the medium. The optimization of inulinase pro¬duction using copra waste as a carbon source was performed with statistical methodology based on experimental designs. The screening of eighteen nut¬rients for their influence on inulinase production was achieved using a Plackett––Burman design. Corn steep liquor, (NH4)2SO4, ZnSO4×7H2O, K2HPO4 and urea were selected based on their positive influence on inulinase production. The selected components were optimized using response surface methodology (RSM). The optimum conditions are: corn steep liquor – 0.0560 (g/gds), (NH4)2SO4 – 0.0084 (g/gds), ZnSO4×7H2O – 0.0254 (g/gds), K2HPO4 – 0.0037 (g/gds) and urea - 0.02147 (g/gds). These conditions were validated experimentally which revealed an enhanced inulinase yield of 372 U/gds.
关键词：inulinase ; copra waste ; K. marxianus var. marxianus ; optimization ; RSM