文章基本信息

标题：Uso di librerie fagiche per isolare in vitro anticorpi monoclonali ricombinanti di predeterminata specificità
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作者：Michela FLEGO (a) ; Alessandro ASCIONE (a) ; Alessandro PINI (b) 等
期刊名称：Annali dell'Istituto Superiore di Sanita
印刷版ISSN：0021-2571
出版年度：2002
卷号：38
期号：04
出版社：Editrice Kurtis Srl
摘要：Summary (Use of phage libraries for the in vitro production of recombinant monoclonal antibodies of predetermined specificity). - The biotechnological generation of monoclonal antibodies of predetermined specificity has traditionally involved the production of hybridomas obtained by somatic cellular fusion of splenocytes from immunized animals with myeloma cell lines bearing selectable markers. Now, monoclonal antibodies could be genetically engineered thus bypassing all the natural systems for making antibodies. Filamentous bacteriophages provides a means to display and select large single chain fragments variable (scFv) repertoires created by cloning the natural rearranged V-immunoglobulin genes or introducing predetermined level of randomization into germline V-gene segments. In this article we demonstrated that by using a well characterized scFv phage synthetic library it is possible to generate in vitro recombinant human monoclonal antibodies directed to a large array of antigens showing different molecular weigths, conformations and origins
关键词：Key words: VH-VL fragments, phage library, recombinant antibodies