Fish lipid contains long-chain n-3 (omega-3) PUFA, particularly EPA (C20:5 n-3) and DHA (C22:6 n-3).
Consumptions of these PUFAs have been perceived to be important in human nutrition, health and disease
prevention. World fish lipid request continue to increase. Fish lipid that contributes to the nutritional needs
is currently being extracted from liver or muscle of cod, herring, mackerel and sardine. Sardine, the important
industrial fish, discharged considerable amount of wastes. These wastes include the head, liver and intestine.
Substantial amount of lipid can be extracted from these wastes. All the extracted oils were less than 6 % of
which the highest was in liver (5.80 %). The predominant fatty acids in sardine wastes were palmitic
(C16:0; 27.80- 35.56 %), stearic (C18:0; 5.90- 9.30 %), oleic (C18:1c; 15.47- 21.79 %) and docosahexaenoic acid
(DHA; C22:6; 11.87- 15.95 %). The n3 / n6 ratio of the respective head, liver and intestine lipid samples showed
the value higher than 1. Due to n-3 fatty acid compound and n-3 / n-6 ratio, lipid from sardine waste may be a
valuable source for human consumption.