文章基本信息

标题：Simulation of 1D and 2D Electrophoretic Separations in Microfluidics Chips
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作者：Pablo A. Kler ; Fabio A. Guarnieri ; Claudio L. A. Berli 等
期刊名称：Mecánica Computacional
印刷版ISSN：2591-3522
出版年度：2009
卷号：XXVIII
期号：24
页码：2053-2070
出版社：CIMEC-INTEC-CONICET-UNL
摘要：Electrophoretic separations comprise a group of analytical techniques such as capillary zone electrophoresis (CZE), isoelectric focusing (IEF), isotachophoresis (ITP) and free ﬂow electrophoresis (FFE). In all cases, separation is based on the dissimilar mobility of ionic species under the action of an external electric ﬁeld. These techniques, which are widely used in chemical and biochemical analysis, have been miniaturized in the last years and now represent one of the most important applications of the lab-on-a-chip technology. In a previous work, a generalized numerical model of electrophoresis on microﬂuidic devices was presented. The model is based on the set of equations that governs electrical phenomena (Poisson equation), ﬂuid dynamics (Navier-Stokes equations), mass transport (Nerst-Planck equation) and chemical reactions. Also the relationship between the buffer characteristics (ionic strength, pH) and surface potential of channel walls is taken into consideration. In this work, three application examples are presented: (a) an IEF assay with immobilized pH gradient (IPG) including the inﬂuence of electro-osmotic ﬂow on its performance, (b) an IEF assay involving ampholyte-based pH gradient, and (c) a 2D electrophoresis, involving FFIEF plus CZE. The numerical simulation is carried out by using PETSc-FEM (Portable Extensible Toolkit for Scientiﬁc Computation - Finite Elements Method), in a Python environment developed at CIMEC using high performance parallel computing and solving techniques based on domain decomposition methods.