文章基本信息

标题：The interactions of carbon black particles with reduced glutathione (GSH) and proteins in respiratory tract lining fluid
本地全文：下载
作者：Henryk Zieliński
期刊名称：Journal of Preventive Medicine
印刷版ISSN：1582-5388
出版年度：2001
卷号：9
期号：2
出版社：Institute of Public Health, Iasi
摘要：In this study we examined the impact of carbon black particles (CBPs) type M120 on the reduced glutathione and proteins in the respiratory tract epithelial lining fluid (ELF) using a model exposure system. Model ELF solutions without (-) and with (+) chelating agents were incubated with 500 m g/mL of CBPs in 1 mL aliquots (37^oC) for 0-6h under mixed conditions. At set intervals throughout the exposure, CBPs were removed by centrifugation, and samples rapidly frozen prior to reduced glutathione (GSH), proteins and protein thiols determination. It has been demonstrated that both albumin and chelating agents can protect oxidation of reduced glutathione induced by carbon black particles.
关键词： air pollutants, respiratory tract lining fluid, albumin, reduced glutathione, proteins thiols