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标题：OCUPATIONAL EXPOSURE TO ORGANIC DUST, MICRORGANISMS, ENDOTOXIN AND PEPTIDOGLYCAN AMONG PLANTS PROCESING WORKERS IN POLAND
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作者：Anna Gora ; Barbara Mackiewicz ; Pawel Krawczyk 等
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2009
卷号：16
期号：01
页码：143-143
出版社：Institute of Agricultural Medicine in Lublin
摘要：The objective of present work was to determine and compare the components of bioaerosol in several sectors of plant processing industries. The study was conducted in 10 facilities engaged in herb and grain processing, fl ax threshing, grain storing, baking, and cereals production. The air samples were taken on glass fi bre fi lters with an AS-50 sampler. We determined the concentrations of airborne microorganisms, dust, endotoxin and peptidoglycan. Total concentrations of viable airborne microorganisms ranged from 0.18–861.4 × 103 cfu/m3. The highest levels of microbial contamination of the air were observed at fl ax farms, in grain elevators and in a herb processing plant. Gram-positive bacteria and fungi were detected at all sampling sites and their median concentrations were respectively 18.1 × 103 cfu/m3 and 0.66 × 103 cfu/m3. The concentration of Gramnegative bacteria ranged from 0.0–168.0 × 103 cfu/m3. The concentration of thermophilic actinomycetes ranged from 0.0–1.45 × 103 cfu/m3. Qualitatively, Gram-positive bacteria constituted 23–93% of the total microbial count. The most common species were: Staphylococcus spp., Curtobacterium pusillum, Rhodococcus fascians, Aureobacterium testaceum, Sanguibacter keddieii, Microbacterium spp., and Bacillus spp. Gram-negative bacteria formed 0–48% of the total count. The species Pantoea agglomerans dominated in all examined air samples. Fungi constituted 2.5–76.9% of the total microbial count. Among them, Penicillium spp., Mucor spp., Alternaria spp., Aspergillus niger, and Aspergillus spp. were found. The dust concentration ranged from 0.18–86.9 mg/m3. The concentration of endotoxin was large and ranged from 0.0041–1562.6 μg/m3. Muramic acid, the chemical marker of peptidoglycan, was detected in 9 out of 13 (69.2%) collected samples. The concentration of peptidoglycan ranged from 1.93–416 ng/m3. A highly signifi cant correlation was found between the individual components of bioaerosol determined in this study. The concentration of endotoxin was correlated with the concentration of Gram-negative bacteria, total microorganisms, and peptidoglycan (R>0.9, p<0.001). The concentration of peptidoglycan was correlated with the concentration of Gram-positive bacteria, Gram-negative bacteria, and total microorganisms (R>0.9, p<0.001).
关键词：organic dust, microorganisms, endotoxin, peptidoglycan, bioaerosols, agricultural industry, plant processing.