文章基本信息

标题：Detection of Brucella by Peripheral Blood PCR and Comparison with Culture and Serological Methods in Suspected Cases
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作者：B Kazemi ، SA Yousefi Namin ، M Bandepour ، F Kafilzadeh ، L Gachkar ، F Mahmoudinejad ، A Samarghandi ، M Mardani
期刊名称：Iranian Journal of Public Health
印刷版ISSN：2251-6085
电子版ISSN：2251-6093
出版年度：2008
卷号：37
期号：04
页码：96-102
出版社：Tehran University of Medical Sciences
摘要：Background: Brucellosis is a zoonotic disease and its symptoms and appearances are not exclusive in human and its traditional diagnosis is based on blood culture and serological methods. For more sensitive and specific detection, the PCR method is recommended.
Methods: One hundred four blood samples were gathered from suspicious patients with brucellosis different ages form Kermanshah, Mazandaran, Khorassan and Hormozgan provinces, and were examined by Rose Bengal, anti globulin, culture and PCR methods.
Results: Seventy three samples were positive by PCR method, 15 samples were positive by cultured method and 84 samples were positive by serological methods.
Conclusion: PCR method is sensitive and specific for diagnosis of Brucella from peripheral blood in suspected cases
关键词：Brucella ، Culture and serological methods ، PCR