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标题：Effects of the heterocyclic amine 2-amino-3,8-dimethylimidazo(4,5-f)quinoxaline (MeIQx) on human lymphocyte DNA in diabetic and non-diabetic population sub-groups: an assessment using the Comet Assay. Susceptibility in human lymphocytes to MeIQx23
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作者：Natalie Wyatt ; Christine Kelly,Vincenzo,Fontana ; Domenico Franco Merlo 等
期刊名称：Journal of Preventive Medicine
印刷版ISSN：1582-5388
出版年度：2005
卷号：13
期号：3-4
出版社：Institute of Public Health, Iasi
摘要：2-amino-3,8-dimethylimidazo(4,5-f)quinoxaline (MeIQx) is one of the most abundant heterocyclic amines (HAs) in cooked food, and has been subjected to numerous toxicological procedures. Our aim was to assess the extent of DNA damage induced by MeIQx in lymphocytes in the alkaline Comet assay, and to compare various population sub-groups. Materials and methods . The alkaline Comet assay was used to investigate lymphocytes from 63 individuals (diabetic and non-diabetic, different ethnicities, sexes, ages, diets, etc) in vitro. Results . No statistically significant differences in susceptibility to DNA damage were observed between subject covariates using linear regression (multivariate analysis) techniques. However, using non-parametric data analysis, statistically significant differences were seen between groups above and below 45 years of age. Conclusions . MeIQx produced highly statistically significant, yet relatively low levels of alkali labile sites, and single strand breaks in DNA in lymphocytes. There were no differences in susceptibility in population sub-groups to this dietary compound.
关键词：Heterocyclic amines: 2-amino-3,8-dimethylimidazo(4,5-f)quinoxaline, MeIQx, Comet Assay, susceptibility