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标题：Health Patterns and Determinants Lead exposure in mothers and children from an industrial region of Poland: an assessment of micronuclei using fluorescence in situ hybridisation. Does lead contribute to increased micronuclei levels?
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作者：Natalie P Wyatt ; Lucyna Kapka ; Adolf Baumgartner 等
期刊名称：Journal of Preventive Medicine
印刷版ISSN：1582-5388
出版年度：2005
卷号：13
期号：3-4
出版社：Institute of Public Health, Iasi
摘要：To investigate the susceptibility to micronuclei in lead-exposed mother and child groups employing fluorescence in situ hybridisation on micronuclei using pan-centromeric DNA probes (MN-FISH assay). Materials and Methods . DOP-PCR (degenerate oligonucleotide primed polymerase chain reaction)was used to generate a pan-centromeric DNA probe. After labelling, the probe was applied to slides with micronuclei smears, allowing the micronuclei to be examined for the presence of centromeric signals. Results . Nine year–old children (n=11) had a significantly lower (p<0.01) level of micronuclei (both with, and without centromeric signals) in comparison with mothers (n=11), and a significantly lower level of centromere negative micronuclei was observed in the children (p<0.01). There was also a lower non-significant level of centromere positive micronuclei induced in comparison with centromere negative micronuclei. Conclusions . The higher rate of micronuclei induced in mothers may arise through lead-exposure and increasing age, and the micronuclei induced arise more from chromosomal fragmentation, than chromosomal malsegregation.
关键词：Lead, micronuclei, FISH, mother-children pairs, susceptibility