文章基本信息

标题：MONITORING LEGIONELLA SPECIES IN HOSPITAL WATER SYSTEMS. LINK WITH DISEASE AND EVALUATION OF DIFERENT DETECTION METHODS
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作者：Maria Kozioł-Montewka ; Agnieszka Magryś ; Nimfa Stojek 等
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2008
卷号：15
期号：01
页码：143-143
出版社：Institute of Agricultural Medicine in Lublin
摘要：The aim of this work was to evaluate three currently available isolation meth- ods for Legionella using water samples and swabs of a single pediatric hospital water system. Additionally, high risk patients were screened for the presence of Legionella pneumophila antigen in urine. Fifteen water samples and 11 swab samples were col- lected from distal sites at 18 sampling locations. The International Standard Method (PN- ISO11731-2) based on membrane ? ltration and direct culture of bacteria on selective media were compared with amoebic co-culture. The numbers of legionellae detected ex- 2 ceeded 10 cfu/100 ml in 50% of the samples. All the positive samples contained L. pneu- mophila SGs 2¨C14. Urine samples were obtained from 57 immunosuppressed children and screened for the presence of L. pneumophila serogroup (SG) 1 antigen by Legionella urinary antigen EIA. Of the 57 urine samples tested for the presence of Legionella pneu- mophila SG 1 antigen, none were positive. Our results highlight the value of combined membrane ? ltration and amoebic co-culture methods in detecting viable L. pneumophila strains. Direct plating of 0.2 ml water is a useful screening method for samples containing
关键词：Legionella, hospital potable water system, Standard PN-ISO11731-2, amoebic co-culture, Legionella urinary antigen, children.