文章基本信息

标题：Epidemiologia molecolare della malaria d’importazione in Italia: l’impiego di marcatori genetici e di saggi di sensibilità in vitro nello studio della clorochino-resistenza in Plasmodium falciparum
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作者：Michela Menegon(a) ; Anna Rosa Sannella(a) ; Carlo Severini(a) 等
期刊名称：Annali dell'Istituto Superiore di Sanita
印刷版ISSN：0021-2571
出版年度：2006
卷号：42
期号：02
页码：203-210
出版社：Editrice Kurtis Srl
摘要：(Molecular epidemiology of imported malaria in Italy: the use of genetic markers and in vitro sensitivity test in a study of chloroquine resistance in Plasmodium falciparum). The emergence of Plasmodium falciparum drug-resistance, especially chloroquine resistance, represents one of the main obstacles to the control of malaria. Several studies have shown that in P. falciparum the mechanism of chloroquine resistance is linked to specific point mutations in the pfcrt gene of the parasite. In the present study we have analyzed 120 Italian imported malaria cases to evaluate the prevalence of 76T and 220S mutantions in the pfcrt gene. Moreover, the correlation between the presence of pfcrt point mutations and in vitro chloroquine resistance has been evaluated on 25 plasmodial isolates. The results showed a high prevalence of the pfcrt point mutations in isolates analyzed and a significant association between point mutations and in vitro chloroquine resistance. Molecular screening on imported malaria cases can be a useful tool to be employed in surveillance activity and also in monitoring the development and spread of drug resistance in endemic areas
关键词：Plasmodium falciparum, drug resistance, chloroquine, pfcrt, molecular epidemiology, Italy, imported malaria.