文章基本信息

标题：Revitalisierung von Säugerzellen nach Einwirkung von Antiseptika
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作者：Müller G, Kramer A
期刊名称：GMS Krankenhaushygiene Interdisziplinär
印刷版ISSN：1863-5245
出版年度：2007
卷号：02
期号：02
出版社：German Medical Science GMS Publishing House, Düsseldorf
摘要：The cytotoxic activity of the antiseptics octenidine dihydrochloride (OCT), polihexanid (PHMB), and povidone iodine (PVP-I) were investigated on epithelial cells (CHO-K1, ATCC CCL61) and fibroblasts (L929, ATCC CCL1) both immediately after 30 min exposure and after 30 min exposure followed by a culture after 24 h without active agents and compared with chlorhexidine digluconate (CHX), and silver-containing formulations. The cytotoxic reaction was detected using MTT assay (intracellular cytotoxicity) and neutral red (NR) assay (damage of the cell membrane). Concentrations of PVP-I resulting after 30 min in >20% vitality revitalize after 24 h of culture without PVP-I. The cytotoxic effect of PHMB is only partially reversible in the cell membrane cytotoxicity at concentrations below the IC50 (vitality >50%). A total revitalization was impossible, because simutaneously the intracellular cytotoxicity increases during the 24 h culture. The cytotoxic effect of OCT, CHX, and silver-containing formulations is irreversible, but only the cell-bound OCT remains biologically activity against microorganisms.
关键词：revitalization of cells, octenidine dihydrochloride, polihexanide, chlorhexidine digluconate, silver-based antiseptics