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标题：Antibakterielle Wirkung von Fliegenmaden (L. sericata) in vitro
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作者：Daeschlein G ; Hoffmeister B ; Below H 等
期刊名称：GMS Krankenhaushygiene Interdisziplinär
印刷版ISSN：1863-5245
出版年度：2006
卷号：01
期号：01
出版社：German Medical Science GMS Publishing House, Düsseldorf
摘要：Maggots of Lucilia sericata are used as an alternative to surgical intervention and long-term antiseptic therapy for the treatment of chronic wounds. To quantify the bactericidal effect of secretions from larvae, an in-vitro test model based on the modified European quantitative suspension test (EN 1040) was developed, in which a co-culture of maggots and bacteria (Micrococcus luteus, Escherichia coli, Methicillin-sensitive Staphylococcus aureus) in tryptic soy broth was tested. The numbers of bacterial colonies with and without maggot exposure were compared after 24, 48, and 72 h of exposure. The mean log10 reduction factor (RF) for bacterial elimination per maggot was > 4 at all examined times for all tested bacteria. Thus, maggot secretion fulfilled the required definitions of an antiseptic. In addition, maggot's ability of ingesting bacteria was also evaluated. Maggots contained viable bacteria after 48 h of contact with the respective organisms. These maggots also continued excreting bacteria.