文章基本信息

标题：Appraisal of hepatic lipase and lipoprotein lipase activities in mice
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作者：Dallinga-Thie, G. M. ; Zonneveld-de Boer, A. J. ; van Vark-van der Zee, L. C. 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2007
卷号：48
期号：12
页码：2788-2791
DOI：10.1194/jlr.D700021-JLR200
出版社：American Society for Biochemistry and Molecular Biology
摘要：A variety of methods are currently used to analyze HL and LPLactivities in mice. In search of a simple methodology, we analyzedmouse preheparin and postheparin plasma LPL and HL activitiesusing specific polyclonal antibodies raised in rabbit againstrat HL (anti-HL) and in goat against rat LPL (anti-LPL). Asan alternative, we analyzed HL activity in the presence of 1M NaCl, a condition known to inhibit LPL activity in humans.The assays were validated using plasma samples from wild-typeand HL-deficient C57BL/6 mice. We now show that the use of 1M NaCl for the inhibition of plasma LPL activity in mice maygenerate incorrect measurements of both LPL and HL activities.Our data indicate that HL can be measured directly, withoutheparin injection, in preheparin plasma, because virtually allHL is present in an unbound form circulating in plasma. In contrast,measurable LPL activity is present only in postheparin plasma.Both HL and LPL can be measured using the same assay conditions(low salt and the presence of apolipoprotein C-II as an LPLactivator). Total lipase activity in postheparin plasma minuspreheparin HL activity reflects LPL activity. Specific antibodiesare not required. Supplementary key words high salt • preheparin plasma • postheparin plasma