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标题：Identification and quantification of dolichol and dolichoic acid in neuromelanin from substantia nigra of the human brain
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作者：Ward, Weslyn C. ; Guan, Ziqiang ; Zucca, Fabio A. 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2007
卷号：48
期号：7
页码：1457-1462
DOI：10.1194/jlr.C700008-JLR200
出版社：American Society for Biochemistry and Molecular Biology
摘要：Neuromelanin (NM) isolated from the substantia nigra of thehuman brain is found to contain a series of dolichoic acids(dol-CA) containing 14–20 isoprene units. This is thefirst observation of dol-CA in a natural system. Using internallyspiked nor-dolichol and nor-dolichoic acid standards, the concentrationsof dolichol (dol) and dol-CA present in NM were determined.Remarkably, dol was only four times as abundant as dol-CA inNM. The distribution of dol-CA chains lengths in NM also differedfrom that of dol, suggesting that the enzyme(s) responsiblefor the conversion of dol to dol-CA prefer a dolichol substratecontaining 19 isoprene units. Supplementary key words dolichyl • isoprenoid • Parkinson's disease • melanin Abbreviations: dol, dolichol; dol-CA, dolichoic acid; LC/MS, liquid chromatography-mass spectrometry; NM, neuromelanin; SN, substantia nigra