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标题：Endothelial lipase is less effective at influencing HDL metabolism in vivo in mice expressing apoA-II
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作者：Broedl, Uli C. ; Jin, Weijun ; Fuki, Ilia V. 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2006
卷号：47
期号：10
页码：2191-2197
DOI：10.1194/jlr.M600036-JLR200
出版社：American Society for Biochemistry and Molecular Biology
摘要：Endothelial lipase (EL) plays an important physiological rolein modulating HDL metabolism. Data suggest that plasma containsan inhibitor of EL, and previous studies have suggested thatapolipoprotein A-II (apoA-II) inhibits the activity of severalenzymes involved in HDL metabolism. Therefore, we hypothesizedthat apoA-II may reduce the ability of EL to influence HDL metabolism.To test this hypothesis, we determined the effect of EL expressionon plasma phospholipase activity and HDL metabolism in humanapoA-I and human apoA-I/A-II transgenic mice. Expression ofEL in vivo resulted in lower plasma phospholipase activity andsignificantly less reduction of HDL-cholesterol, phospholipid,and apoA-I levels in apoA-I/A-II double transgenic mice comparedwith apoA-I single transgenic mice. We conclude that the presenceof apoA-II on HDL particles inhibits the ability of EL to influencethe metabolism of HDL in vivo. Supplementary key words high density lipoprotein metabolism • apolipoprotein A-II • high density lipoprotein containing apolipoprotein A-I but no apolipoprotein A-II • high density lipoprotein containing both apolipoprotein A-I and apolipoprotein A-II • transgenic mice Abbreviations: AdGFP, adenovirus encoding green fluorescent protein; AdhEL, adenovirus encoding human endothelial lipase; apoA-I, apolipoprotein A-I; DPPC, dipalmitoylphosphatidylcholine; EL, endothelial lipase; HDL-C, high density lipoprotein-cholesterol; HSPG, heparan sulfate proteoglycan; LpA-I, high density lipoprotein containing apolipoprotein A-I but no apolipoprotein A-II; LpA-I/A-II, high density lipoprotein containing both apolipoprotein A-I and apolipoprotein A-II