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标题：Vitamin A combined with retinoic acid increases retinol uptake and lung retinyl ester formation in a synergistic manner in neonatal rats
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作者：Ross, A. Catharine ; Ambalavanan, Namasivayam ; Zolfaghari, Reza 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2006
卷号：47
期号：8
页码：1844-1851
DOI：10.1194/jlr.M600061-JLR200
出版社：American Society for Biochemistry and Molecular Biology
摘要：Vitamin A (VA) is stored in tissues predominantly as retinylesters (REs), which provide substrate for the production ofbioactive retinoids. Retinoic acid (RA), a principal metabolite,has been shown to induce postnatal lung development. To betterunderstand lung RE storage, we compared VA (given as retinylpalmitate), RA, and a nutrient-metabolite combination, VARA,given orally on postnatal days 5–7, for their abilityto increase lung RE in neonatal rats. VARA increased lung REsignificantly [ $~$ 14, 2.4, 2.1, and <1 nmol/g for VARA, VA,RA, and control (C), respectively; P < 0.001]; the increaseby VARA was more than additive compared with the effects ofVA and RA alone. Lung histology and morphometry were unchanged.In a 6 h metabolic study, providing [³H]retinol with VARA, comparedwith VA or C, increased the uptake of newly absorbed ³H by 3-fold,indicating that VARA stimulated the uptake of [³H]retinol andits retention as [³H]RE in neonatal lungs. After cessation ofVARA, lung RE remained increased for 9 d afterward, throughthe period of alveolar development. In conclusion, VARA, a 10:1nutrient-metabolite combination, increased lung RE significantlycompared with VA alone and could be a promising therapeuticoption for enhancing the delivery of VA to the lungs. Supplementary key words lung retinyl ester storage • retinol esterification • liver retinyl ester • newly absorbed retinol • neonatal vitamin A status Abbreviations: RA, retinoic acid; RE, retinyl ester; VA, vitamin A