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标题：Absence of VLDL secretion does not affect {alpha}-tocopherol content in peripheral tissues
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作者：Minehira-Castelli, Kaori ; Leonard, Scott W. ; Walker, Quinn M. 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2006
卷号：47
期号：8
页码：1733-1738
DOI：10.1194/jlr.M600125-JLR200
出版社：American Society for Biochemistry and Molecular Biology
摘要： ${alpha}$ -Tocopherol is a lipid-soluble antioxidant that helps to preventoxidative damage to cellular lipids. ${alpha}$ -Tocopherol is absorbedby the intestine and is taken up and retained by the liver;it is widely presumed that ${alpha}$ -tocopherol is then delivered toperipheral tissues by the secretion of VLDL. To determine whetherVLDL secretion is truly important for the delivery of ${alpha}$ -tocopherolto peripheral tissues, we examined ${alpha}$ -tocopherol metabolism inmice that lack microsomal triglyceride transfer protein (Mttp)expression in the liver and therefore cannot secrete VLDL (Mttp^/mice). Mttp^/ mice have low plasma lipid levels and increasedstores of lipids in the liver. Similarly, ${alpha}$ -tocopherol levelsin the plasma were lower in Mttp^/ mice than in controls, whereashepatic ${alpha}$ -tocopherol stores were higher. However, ${alpha}$ -tocopherollevels in the peripheral tissues of Mttp^/ mice were nearly identicalto those of control mice, suggesting that VLDL secretion isnot critical for the delivery of ${alpha}$ -tocopherol to peripheral tissues.When fed a diet containing deuterated ${alpha}$ -tocopherol, Mttp^/ andcontrol mice had similar incorporation of deuterated ${alpha}$ -tocopherolinto plasma and various peripheral tissues. We conclude thatthe absence of VLDL secretion has little effect on the storesof ${alpha}$ -tocopherol in peripheral tissues, at least in the mouse. Supplementary key words ${gamma}$ -tocopherol • microsomal triglyceride transfer protein • vitamin E Abbreviations: apoB, apolipoprotein B; Mttp, microsomal triglyceride transfer protein; pI-pC, polyinosinic-polycytidylic ribonucleic acid; ${alpha}$ -TTP, ${alpha}$ -tocopherol transfer protein