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标题：Effects of diet and metamorphosis upon the sterol composition of the butterfly Morpho peleides
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作者：Connor, William E. ; Wang, Yingming ; Green, Mike 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2006
卷号：47
期号：7
页码：1444-1448
DOI：10.1194/jlr.M600056-JLR200
出版社：American Society for Biochemistry and Molecular Biology
摘要：Whole body sterol metabolism in insects has seldom been studied.We were able to design an appropriate study at a butterfly farmin Belize. We collected six larvas of butterfly (Morpho peleides),their food (leaves of Pterocarpus bayessii), and their excretions.In addition, six adult butterflies were collected. The sterolsof the diet, the larva, and adult butterfly were analyzed bygas-liquid chromatography. The structures of these sterols wereidentified by digitonin precipitation, GC-MS, and NMR. Foursterols (cholesterol, campesterol, stigmasterol, and sitosterol)and a sterol mixture were found in the food, the body, and theexcreta of the larva. The tissue sterol content of the larvawas 326 µg. They consumed 276 µg of sterols perday. Their excretion was 185 µg per day as sterols. Thetotal tissue sterol contents of the larva and butterfly weresimilar, but they had different sterol compositions, which indicatedinterconversion of sterols during development. There was a progressiveincrease in the cholesterol content from larva to butterflyand a decrease in the content of sitosterol and other plantsterols, which were likely converted to cholesterol. Our dataindicated an active sterol metabolism in butterfly larva. Dietplayed an important role in determining its sterol composition.During metamorphosis, there was an interconversion of sterols.This is the first paper documenting the fecal sterol excretionin insects as related to dietary intakes. Supplementary key words cholesterol • plant sterols • sitosterol • campesterol • dealkylation • larva • adult butterfly • tissue sterols • fecal sterols