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标题：Synthesis of long-chain polyunsaturated fatty acids in lactating mammary gland: role of {Delta}5 and {Delta}6 desaturases, SREBP-1, PPAR{alpha}, and PGC-1
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作者：Rodriguez-Cruz, Maricela ; Tovar, Armando R. ; Palacios-Gonzalez, Berenice 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2006
卷号：47
期号：3
页码：553-560
DOI：10.1194/jlr.M500407-JLR200
出版社：American Society for Biochemistry and Molecular Biology
摘要：The purpose of this work was to study whether rat lactatingmammary gland can synthesize PUFAs through the expression of ${Delta}$ 5 and ${Delta}$ 6 desaturases ( ${Delta}$ 5D and ${Delta}$ 6D), whether these enzymes are regulatedby the transcription factors sterol-regulatory element bindingprotein 1 (SREBP-1) and peroxisome proliferator-activated receptor ${alpha}$ (PPAR ${alpha}$ ) and the coactivator peroxisome proliferator-activatedreceptor ${gamma}$ coactivator 1ß (PGC-1ß), and whetherthese desaturases are regulated by the lipid concentration inthe diet. The results showed that on day 12 of lactation, $~$ 35%of the linoleic acid in the diet, which is the precursor ofPUFAs, is transferred to the mammary gland. There was expressionof ${Delta}$ 5D and ${Delta}$ 6D in mammary gland, and it was regulated by the cornoil content in the diet. The higher the corn oil content inthe diet, the lower the expression of both desaturases. Inductionof ${Delta}$ 5D and ${Delta}$ 6D was associated positively with similar changesin SREBP-1 and PGC-1ß. Expression of PPAR ${alpha}$ was barelydetected and was not affected by the corn oil content in thediet, whereas PGC-1ß expression increased as the cornoil in the diet increased. These results indicate that the lactatingmammary gland has the capacity to synthesize PUFAs and can beregulated by the lipid content in the diet. Supplementary key words sterol-regulatory element binding protein 1 • peroxisome proliferator-activated receptor ${alpha}$ • peroxisome proliferator-activated receptor ${gamma}$ coactivator 1