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标题：Eicosapentaenoic acid (20:5 n-3) increases fatty acid and glucose uptake in cultured human skeletal muscle cells
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作者：Aas, Vigdis ; Rokling-Andersen, Merethe H. ; Kase, Eili Tranheim 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2006
卷号：47
期号：2
页码：366-374
DOI：10.1194/jlr.M500300-JLR200
出版社：American Society for Biochemistry and Molecular Biology
摘要：This study was conducted to evaluate the chronic effects ofeicosapentaenoic acid (EPA) on fatty acid and glucose metabolismin human skeletal muscle cells. Uptake of [¹⁴C]oleate was increased>2-fold after preincubation of myotubes with 0.6 mM EPA for24 h, and incorporation into various lipid classes showed thatcellular triacylgycerol (TAG) and phospholipids were increased2- to 3-fold compared with control cells. After exposure tooleic acid (OA), TAG was increased 2-fold. Insulin (100 nM)further increased the incorporation of [¹⁴C]oleate into alllipid classes for EPA-treated myotubes. Fatty acid ß-oxidationwas unchanged, and complete oxidation (CO₂) decreased in EPA-treatedcells. Basal glucose transport and oxidation (CO₂) were increased2-fold after EPA, and insulin (100 nM) stimulated glucose transportand oxidation similarly in control and EPA-treated myotubes,whereas these responses to insulin were abolished after OA treatment.Lower concentrations of EPA (0.1 mM) also increased fatty acidand glucose uptake. CD36/FAT (fatty acid transporter) mRNA expressionwas increased after EPA and OA treatment compared with controlcells. Moreover, GLUT1 expression was increased 2.5-fold byEPA, whereas GLUT4 expression was unchanged, and activitiesof the mitogen-activated protein kinase p38 and extracellularsignal-regulated kinase were decreased after treatment withOA compared with EPA. Together, our data show that chronic exposureof myotubes to EPA promotes increased uptake and oxidation ofglucose despite a markedly increased fatty acid uptake and synthesisof complex lipids. Supplementary key words human skeletal myotubes • lipid metabolism • glucose metabolism Abbreviations: ASM, acid-soluble metabolite; BSA, bovine serum albumin; EPA, eicosapentaenoic acid; ERK, extracellular signal-regulated kinase; FCS, fetal calf serum; ${alpha}$ MEM, minimum essential medium ${alpha}$ ; OA, oleic acid; PKB, protein kinase B; PL, phospholipid; PPAR ${gamma}$ , peroxisome proliferator-activated receptor ${gamma}$ ; TAG, triacylglycerol