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标题：Ion-trap tandem mass spectrometric analysis of Amadori-glycated phosphatidylethanolamine in human plasma with or without diabetes
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作者：Nakagawa, Kiyotaka ; Oak, Jeong-Ho ; Higuchi, Ohki 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2005
卷号：46
期号：11
页码：2514-2524
DOI：10.1194/jlr.D500025-JLR200
出版社：American Society for Biochemistry and Molecular Biology
摘要：Peroxidized phospholipid-mediated cytotoxicity is involved inthe pathophysiology of diseases [i.e., an abnormal increaseof phosphatidylcholine hydroperoxide (PCOOH) in plasma of type2 diabetic patients]. The PCOOH accumulation may relate to Amadori-glycatedphosphatidylethanolamine (Amadori-PE; deoxy-D-fructosyl phosphatidylethanolamine),because Amadori-PE causes oxidative stress. However, the occurrenceof lipid glycation products, including Amadori-PE, in vivo isstill unclear. Consequently, we developed an analysis methodof Amadori-PE using a quadrupole/linear ion-trap mass spectrometer,the Applied Biosystems QTRAP. In positive ion mode, collision-induceddissociation of Amadori-PE produced a well-characterized diglycerideion ([M+H–303]⁺) permitting neutral loss scanning andmultiple reaction monitoring (MRM). When lipid extract fromdiabetic plasma was infused directly into the QTRAP, Amadori-PEmolecular species could be screened out by neutral loss scanning.Interfacing liquid chromatography with QTRAP mass spectrometryenabled the separation and determination of predominant plasmaAmadori-PE species with sensitivity of $~$ 0.1 pmol/injection inMRM. The plasma Amadori-PE level was 0.08 mol% of total PE inhealthy subjects and 0.15–0.29 mol% in diabetic patients.Furthermore, plasma Amadori-PE levels were positively correlatedwith PCOOH (a maker for oxidative stress). These results show the involvement between lipid glycation andlipid peroxidation in diabetes pathogenesis. Abbreviations: AGE, advanced glycation end product; Amadori-PE, Amadori-glycated phosphatidylethanolamine; Hb_A1c, hemoglobin A1c; LC-MS/MS, liquid chromatography-tandem mass spectrometry; MRM, multiple reaction monitoring; PCOOH, phosphatidylcholine hydroperoxide; PE, phosphatidylethanolamine; PEOOH phosphatidylethanolamine hydroperoxide; PS, phosphatidylserine; QqLIT, hybrid quadrupole/linear ion trap Supplementary key words glycation • QTRAP mass spectrometer • lipid peroxidation