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标题：Determining hepatic triglyceride production in mice: comparison of poloxamer 407 with Triton WR-1339
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作者：Millar, John S. ; Cromley, Debra A. ; McCoy, Mary G. 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2005
卷号：46
期号：09
页码：2023-2028
DOI：10.1194/jlr.D500019-JLR200
出版社：American Society for Biochemistry and Molecular Biology
摘要：Triglyceride (TG), a water-insoluble energy-rich lipid, is secretedby the liver as part of very low density lipoproteins (VLDLs)to supply energy to extrahepatic tissues. Overproduction ofVLDL is associated with increased risk of cardiovascular heartdisease; this has renewed an interest in factors that affecthepatic TG production. The TG production rate is determinedby measuring temporal increases in plasma TG under conditionsin which TG hydrolysis by lipoprotein lipase (LPL) is inhibited.The nonionic detergent, Triton WR-1339 (Triton), has commonlybeen used to inhibit LPL for this purpose. Triton, in additionto inhibition of TG hydrolysis, has properties that have thepotential to adversely influence lipoprotein metabolism. Anothernonionic detergent, poloxamer 407 (P-407), also inhibits LPL. In these studies, we demonstrate that P-407 is comparable toTriton in the determination of TG production but without theunwanted side effects of Triton. Abbreviations: apoA-I, apolipoprotein A-I; FPLC, fast-protein liquid chromatography; HLB, hydrophilic/lipophilic balance; LPL, lipoprotein lipase; P-407, poloxamer 407; TG, triglyceride Supplementary key words non-ionic detergents • hepatic lipids • lipoproteins