文章基本信息

标题：A proteomic study of the apolipoproteins in LDL subclasses in patients with the metabolic syndrome and type 2 diabetes
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作者：Davidsson, Pia ; Hulthe, Johannes ; Fagerberg, Bjorn 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2005
卷号：46
期号：09
页码：1999-2006
DOI：10.1194/jlr.M500161-JLR200
出版社：American Society for Biochemistry and Molecular Biology
摘要：The exchangeable apolipoproteins present in small, dense LDL(sdLDL) and large, buoyant LDL subclasses were evaluated witha quantitative proteomic approach in patients with the metabolicsyndrome and with type 2 diabetes, both with subclinical atherosclerosisand the B LDL phenotype. The analyses included surface-enhancedlaser adsorption/ionization, time-of-flight mass spectrometry,and subsequent identification by mass spectrometry or immunoblottingand were carried out in LDL subclasses isolated by ultracentrifugationin deuterium oxide gradients with near physiological salt concentrations.The sdLDLs of both types of patients were enriched in apolipoproteinC-III (apoC-III) and were depleted of apoC-I, apoA-I, and apoEcompared with matched healthy controls with the A phenotype.The LDL complexes formed in serum from patients with diabeteswith the arterial proteoglycan (PG) versican were also enrichedin apoC-III. In addition, there was a significant correlationbetween the apoC-III content in sdLDL in patients and the apparentaffinity of their LDLs for arterial versican. The unique distribution of exchangeable apolipoproteins in thesdLDLs of the patients studied, especially high apoC-III, coupledwith the augmented affinity with arterial PGs, may contributeto the strong association of the dyslipidemia of insulin resistancewith increased risk for cardiovascular disease. Supplementary key words mass spectrometry • apolipoprotein C-III • apolipoprotein C-I • apolipoprotein E • apolipoprotein A-I • low density lipoproteins • B phenotype