摘要:Wheat ( Triticum aestivum ) is one of the most important food grain crops in all South Asian countries especially in India and Pakistan. These countries have diversifying soil and climatic conditions inserting measurable effect on nutritional as well as anti-nutritional parameters of wheat. Wheat varieties included in this study are collected from different agro-ecological zones of Pakistan. Myoinositol hexa-phosphate ( phytic acid ) one of major anti-nutritional factors wheat. Phytic acid of collected samples was determined to facilitate the crop breeders and agronomists, so that they would also consider this factor while conducting research works. It was observed that wheat varieties showed different levels of phytic acid at different locations. At one location (Islamabad), a variety (Pari-73) showed the highest value of Phytic acid (1.343%) and at other location (Faisalabad), same variety the showed lowest phytic acid (0.74%). This maximum variability (44%) also indicated that there was significant effect of change in location on phytic acid contents of wheat varieties. It is mainly due to presence of available phosphorus reserves in soil as phytate has direct relation to soil phosphorus.