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标题：Xenon prevents cellular damage in differentiated PC-12 cells exposed to hypoxia
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作者：Christian Petzelt ; Per Blom ; Wolfgang Schmehl 等
期刊名称：BMC Neuroscience
印刷版ISSN：1471-2202
电子版ISSN：1471-2202
出版年度：2004
卷号：5
期号：1
页码：1
DOI：10.1186/1471-2202-5-55
语种：English
出版社：BioMed Central
摘要：Background The neuroprotective effect of xenon has been demonstrated for glutamatergic neurons. In the present study it is investigated if dopaminergic neurons, i.e. nerve-growth-factor differentiated PC-12 cells, are protected as well against hypoxia-induced cell damage in the presence of xenon. Results Pheochromocytoma cells differentiated by addition of nerve growth factor were placed in a N₂-saturated atmosphere, a treatment that induced release of dopamine, reaching a maximum after 30 min. By determining extracellular lactate dehydrogenase concentration as marker for concomitant cellular damage, a substantial increase of enzymatic activity was found for N₂-treated cells. Replacement of N₂ by xenon in such a hypoxic atmosphere resulted in complete protection against cellular damage and prevention of hypoxia-induced dopamine release. Intracellular buffering of Ca²⁺ using the Ca-chelator 1, 2- bis (2-Aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis (acetoxymethyl) ester (BAPTA) reduced the neuroprotective effect of xenon indicating the essential participation of intracellular Ca²⁺-ions in the process of xenon-induced neuroprotection. Conclusions The results presented demonstrate the outstanding property of xenon to protect neuron-like cells in a hypoxic situation.