文章基本信息

标题：Pattern of distribution and cycling of SLOB, Slowpoke channel binding protein, in Drosophila
本地全文：下载
作者：Angela M Jaramillo ; Xiangzhong Zheng ; Yi Zhou 等
期刊名称：BMC Neuroscience
印刷版ISSN：1471-2202
电子版ISSN：1471-2202
出版年度：2004
卷号：5
期号：1
页码：1
DOI：10.1186/1471-2202-5-3
语种：English
出版社：BioMed Central
摘要：Background SLOB binds to and modulates the activity of the Drosophila Slowpoke (dSlo) calcium activated potassium channel. Recent microarray analyses demonstrated circadian cycling of slob mRNA. Results We report the mRNA and protein expression pattern of slob in Drosophila heads. slob transcript is present in the photoreceptors, optic lobe, pars intercerebralis (PI) neurons and surrounding brain cortex. SLOB protein exhibits a similar distribution pattern, and we show that it cycles in Drosophila heads, in photoreceptor cells and in neurosecretory cells of the PI. The cycling of SLOB is altered in various clock gene mutants, and SLOB is expressed in ectopic locations in tim ⁰¹flies. We also demonstrate that SLOB no longer cycles in the PI neurons of Clk ^jrkflies, and that SLOB expression is reduced in the PI neurons of flies that lack pigment dispersing factor (PDF), a neuropeptide secreted by clock cells. Conclusions These data are consistent with the idea that SLOB may participate in one or more circadian pathways in Drosophila .