文章基本信息

标题：Synthesis and Bioconjugation of Gold Nanoparticles as Potential Molecular Probes for Light-Based Imaging Techniques
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作者：Raja Gopal Rayavarapu ; Wilma Petersen ; Constantin Ungureanu 等
期刊名称：International Journal of Biomedical Imaging
印刷版ISSN：1687-4188
电子版ISSN：1687-4196
出版年度：2007
卷号：2007
DOI：10.1155/2007/29817
出版社：Hindawi Publishing Corporation
摘要：We have synthesized and characterized gold nanoparticles (spheres and rods) with optical extinction bands within the “optical imaging window.” The intense plasmon resonant driven absorption and scattering peaks of these nanoparticles make them suitable as contrast agents for optical imaging techniques. Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells. The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface. We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles. Some proposed applications of these potential molecular probes in the field of biomedical imaging are also discussed.