文章基本信息

标题：Distribution and Phylogenetic Characteristics of the Genes Encoding Enzymes Relevant to Methane Oxidation in Oxygen Minimum Zones
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作者：Toru Hayashi ; Hajime Obata ; Toshitaka Gamo 等
期刊名称：Research Journal of Environmental Sciences
印刷版ISSN：1819-3412
电子版ISSN：2152-8238
出版年度：2007
卷号：1
期号：6
页码：275-284
DOI：10.3923/rjes.2007.275.284
出版社：Academic Journals Inc., USA
摘要：A total of 34 pelagic water samples from the upper-, mid-and lower-Oxygen Minimum Zones (OMZs) in the eastern Pacific Ocean were collected. Particles suspended therein were captured by 0.2 μm pore size filters to extract bulk genomic DNA for PCR amplification of the genes relevant to methane oxidation. The genes encoding particulate methane monooxygenase and its relative enzyme ammonia monooxygenase (β), pmoA and amoA (β), respectively, were amplified and analyzed. Five OMZ samples (from four sites) showed amplification of pmoA . This finding provide the bases for consideration that pelagic methane oxidation may occur in OMZs. Interestingly, one pmoA sequence was identical (based on amino acid s) to pmoA of the methanotrophic endosymbiont.