摘要:This investigation was aimed to estimate the heavy metal pollution in marine and fresh water and their acute toxicity and its toxicological affects on survival, physiological and biochemical parameters of the widely consumed fresh water and marine water fishes of Sindh. Four bioindicators, two from marine water ( Liza subviridus and Johnius belengerii ) and two from fresh water species ( Cyprius carpio and Pomodasy argyrew ) were collected to study the species-site interaction. Water samples from both stations collected to analyze the essential and non essential metals and muscles of fish were sampled for metabolic parameters and persistent metals pollutant. Total lipids, proteins, amino acid s and glycogen were estimated by Spectrophotometery whereas Atomic Absorption Spectrophotometery was used for metals detection. Results showed that interaction of metal pollutants vary specie to specie. This showed that pollutants act by changing the structural or biological function of bioindicator. High concentrations of contaminants were found in tissues of fishes collected from marine water as compared to fresh water fishes.