文章基本信息

标题：Bioavailability of Ellagic Acid After Single Dose Administration Using HPLC
本地全文：下载
作者：Abdul-Wahab R. Hamad ; Waleed M. Al-Momani ; Sana Janakat 等
期刊名称：Pakistan Journal of Nutrition
印刷版ISSN：1680-5194
电子版ISSN：1994-7984
出版年度：2009
卷号：8
期号：10
页码：1661-1664
DOI：10.3923/pjn.2009.1661.1664
出版社：Asian Network for Scientific Information
摘要：Ellagic Acid (EA) is a complex planar molecule which demonstrates a variety of anticarcinogenic activities. Ellagic Acid acts as a scavenger to "bind" cancer-causing chemicals, making them inactive. It also has anti-bacterial, anti-viral properties. The concentration of EA was assessed in serum of twenty healthy volunteers over time interval from 0.15, 0.3, 1, 1.30, 2, 3, 6, 8 and 12 h. The serum concentration-time profile is presented after administration of 40 mg of ellagic acid to 20 healthy volunteers. Isocratic reversed phase liquid chromatographic method was developed for determination of low quantity of Ellagic Acid (EA) on reversed phase Lichrospher 100 RP-18, 5 μm (250 x 4.6 mm i.d) column and using methanol as mobile phase. The eluted EA was detected by UV set at 254 nm. Calibration curve for standard EA is linear with correlation coefficient of 0.9997 for detector response plotted against concentration range from 20-1000 ng/ml. The concentration of ellagic acid (C_max) was highest in blood one hour (T_max) after oral administration. It was 200.15±26.66 ng/ml., the mean serum elimination half-life was about 8.4±1.4 h. More than 50% of ellagic acid boud to plasma protein following oral administration, absorption of ionized ellagic acid occurs in stomach and intestine. Therefore, one capsule three times has drug bioavailability through all day.