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标题：Characterization of the Transitory Immune Response in Cows Immunized with RB51 and its Implication on Diagnosis Within Brucellosis Endemic Zones
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作者：Efren Diaz-Aparicio ; Beatriz Arellano-Reynoso ; Enrique Herrera Lopez 等
期刊名称：International Journal of Dairy Science
印刷版ISSN：1811-9743
电子版ISSN：1811-9751
出版年度：2007
卷号：2
期号：4
页码：364-371
DOI：10.3923/ijds.2007.364.371
出版社：Academic Journals Inc., USA
摘要：A dairy herd with 17% Brucellosis prevalence was vaccinated or re-vaccinated with RB51. Five groups of ten cows each were classified according to their response to serologic tests. Additionally, one group with infected animals and one with animals originating from a Brucellosis free herd were used as positive and negative controls. A Radial Immunodiffusion test (RID) was used to confirm results. Indirect ELISA was carried out with LPS of B. abortus 2308 (LPS-S) or LPS of B. abortus RB51 (LPS-R) as primary antigens and anti-IgG1, IgG2, IgM and IgA as secondary antigens. When LPS-S was used as antigen, vaccinated and re-vaccinated cows that were positive to card and Rivanol tests, showed an IgG1-type response. Animals that were infected mainly showed an IgG2 type response. When LPS-R was used, none of the animals showed a significant response. Immunized cows that had contact with the wild-type strain showed humoral immune response that was detected as positive serology tests. Therefore, in endemic zones confirmation tests such as RID, Rivanol with titers above 1:100, or ELISA with LPS-S as antigen and IgG2, are essential to differentiate between animals vaccinated with RB51, which have a transitory immune response , from those that are infected.