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标题：Spirulina platensis Extract Reduces Serum TNF-α,Neutrophils,and Increases Macrophage Count in Skin Incisional Mice Model
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作者：Riski Dwi Utami ; Tri Nur Kristina ; Renni Yuniati 等
期刊名称：IJEMS (Indonesian Journal of Environmental Management and Sustainability)
印刷版ISSN：2598-6260
电子版ISSN：2598-6279
出版年度：2020
卷号：4
期号：2
页码：34-38
DOI：10.26554/ijems.2020.4.2.34-38
语种：English
出版社：ARTS Publishing Corp.
摘要：Spirulina sp. is an alga that has antioxidant and anti-inflammatory potential. Phycocyanin contained in Spirulina platensis can reduce tumor necrosis factor-α levels,neutrophils,and M2 macrophages in inflammation. This research aims to investigate the anti-inflammatory activity of S. platensis in a mice wound model. Thirty-two male Wistar rats were incised and divided into 4 groups. The first therapeutic group (X1) received S. platensis extract at a dose of 500 mg/kgBW/day,and the second therapeutic group received S. platensis extract at a dose of 750 mg/kgBW/day. The negative control group (C1) received a saline solution and the positive control group (C2) received diclofenac sodium 20mg/kgBW/day. Serum TNF-α levels examined by the Enzyme-Linked Immunosorbent Assay method. Neutrophils and M2 macrophages were calculated by tissue biopsy and hematoxylin-eosin (HE) staining. Data analysis was performed with ANOVA test and LSD Post-Hoc Test. The average levels of serum TNF-α on the 14-day were 394.50;180.33;2,980.33;and 607.42 pg/ml for X1,X2,C1 and C2,respectively. The average neutrophils number on the 14-day were 8.00;6.83;14.67;and 11.17 for X1,X2,C1 and C2,respectively. The average macrophage number in the 14-day was 15.50;19.17;6.33;and 11.17 for X1,X2,C1 and C2,respectively. We found significant differences between TNF-α levels,the number of neutrophils,and the number of M2 macrophages. The administration of S. platensis extract at a dose of 750 mg/ kgBW/day reduces serum TNF-α levels,neutrophil count,and increases M2 macrophages in skin incisional mice model.
关键词：Spirulina platensis;anti-inflammatory;TNF-α;neutrophils;macrophages