文章基本信息

标题：High-speed high-resolution laser diodebased photoacoustic microscopy for in vivo microvasculature imaging
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作者：Xiufeng Li ; Victor T C Tsang ; Lei Kang 等
期刊名称：Visual Computing for Industry, Biomedicine, and Art
电子版ISSN：2524-4442
出版年度：2021
卷号：4
页码：1-6
DOI：10.1186/s42492-020-00067-5
语种：English
出版社：SpringerOpen
摘要：Laser diodes (LDs) have been considered as cost-effective and compact excitation sources to overcome the requirement of costly and bulky pulsed laser sources that are commonly used in photoacoustic microscopy (PAM). However, the spatial resolution and/or imaging speed of previously reported LD-based PAM systems have not been optimized simultaneously. In this paper, we developed a high-speed and high-resolution LD-based PAM system using a continuous wave LD, operating at a pulsed mode, with a repetition rate of 30 kHz, as an excitation source. A hybrid scanning mechanism that synchronizes a one-dimensional galvanometer mirror and a two-dimensional motorized stage is applied to achieve a fast imaging capability without signal averaging due to the high signal-tonoise ratio. By optimizing the optical system, a high lateral resolution of 4.8 μm has been achieved. In vivo microvasculature imaging of a mouse ear has been demonstrated to show the high performance of our LD-based PAM system.
关键词：Photoacoustic microscopy;Laser diode;In vivo imaging;Microvasculature imaging